instant structured illumination microscopy isim system Search Results


instant structured illumination microscopy isim  (Spirochrome)
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Spirochrome instant structured illumination microscopy isim
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instant structured illumination microscope  (Visitech Inc)
86
Visitech Inc instant structured illumination microscope
Instant Structured Illumination Microscope, supplied by Visitech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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structured illumination microscope (isim  (Hamamatsu)
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Hamamatsu structured illumination microscope (isim
Structured Illumination Microscope (Isim, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isis 3521 supply agreement  (Eli Lilly)
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Eli Lilly isis 3521 supply agreement
Isis 3521 Supply Agreement, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orcaflash4.0 v3 scmos cameras  (Hamamatsu)
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instantsim isim  (Visitech Inc)
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Visitech Inc instantsim isim
Instantsim Isim, supplied by Visitech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vt-isim system  (Hamamatsu)
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Hamamatsu vt-isim system
Vt Isim System, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isim microscope  (MetaMorph Inc)
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MetaMorph Inc isim microscope
(A-B) <t>iSIM</t> images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM <t>microscope.</t> Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.
Isim Microscope, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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+ activation modality is0  (Intellisync Corporation)
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Intellisync Corporation + activation modality is0
(A-B) <t>iSIM</t> images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM <t>microscope.</t> Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.
+ Activation Modality Is0, supplied by Intellisync Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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visitech isim  (Visitech Inc)
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Visitech Inc visitech isim
(A-B) <t>iSIM</t> images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM <t>microscope.</t> Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.
Visitech Isim, supplied by Visitech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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+ activation modality isi  (Intellisync Corporation)
90
Intellisync Corporation + activation modality isi
(A-B) <t>iSIM</t> images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM <t>microscope.</t> Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.
+ Activation Modality Isi, supplied by Intellisync Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ly900003  (Eli Lilly)
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Eli Lilly ly900003
(A-B) <t>iSIM</t> images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM <t>microscope.</t> Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.
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Image Search Results


(A-B) iSIM images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM microscope. Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.

Journal: bioRxiv

Article Title: Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response

doi: 10.1101/492769

Figure Lengend Snippet: (A-B) iSIM images (see Methods) of PVD neurons (late L3 larvae) labeled with PVD::LifeAct::GFP (green) and PVD::mCherry::UNC-34 (magenta). Merge shown at top right. Insets depict punctate mCherry::UNC-34 in 1°, 2° and 3° PVD dendrites. (B) Note mCherry::UNC-34 puncta at tips (white arrowheads) of 3° dendrites. Scale bars are 5 μm. (C) Frequency (100%) at which tips of 3º dendrites show mCherry::UNC-34 puncta, ***p<0.001, Fischer’s exact test, N = 33 dendrite tips in 9 animals. Both iSIM and Airyscan images were used in this analysis. (D-E) Kymographs of PVD::mCherry::UNC-34 in PVD 3° dendrite captured in the iSIM microscope. Moving (green arrowheads) vs stationary (red arrowheads) mCherry::UNC-34 puncta are denoted. The distal end of the 3° dendrite is indicated by an asterisk and a red dashed arrow points toward the PVD cell soma. (F) Trafficking of mCherry::UNC-34 puncta in the PVD 3° dendrite (enclosed with rectangle of red dashed line) from 4E was analyzed using a spots tracking algorithm (Imaris) (see Methods). The net displacement (µm) of mobile mCh::UNC-34 puncta (N = 8) from position at t = 0 in a 3º dendrite plotted against time with a sampling interval of 2 s. Scale bar is 10 μm.

Article Snippet: Time-lapse movies from the iSIM microscope were deconvolved with metamorph.

Techniques: Labeling, Microscopy, Sampling